RNA immunoprecipitation (RIP) protocol - Abcam.
XTT Cell Proliferation Assay Kit Instruction Manual Catalog Number 30-1011K (1000 Assays). Background Principle of the XTT Assay The XTT cell proliferation assay was first described in 1988 by Scudiero et al. (3) as an effective method to measure cell growth and drug sensitivity in tumor cell lines. XTT is a colorless or slightly yellow compound that when reduced becomes brightly orange.
I'm setting up a MTT assay to evaluate cell proliferation and cytotoxicity of mouse spleen cells after polyclonal and antigen-specific stimulation in 96-well flat-bottomed plates. After 4 hour MTT.
Trypan blue exclusion method is one of the earliest and simplest viability assays. Trypan blue is a negatively charged dye which only stains cells with a compromised cell membrane, hence indicating cell death (26). In contrast, viable cells are absent of trypan blue due to both the cell membrane and dye being negatively charged. b. MTT assay. In addition to dye exclusion, some viability assays.
Virus Plaque Assay Protocol This is our preferred protocol which we use routinely at VIRAPUR to perform plaque titration and agarose overlay assays. The technique is applicable to multiple virus systems, and we have used it successfully with many human and animal cytopathic viruses. If you want to outsource this assay, contact VIRAPUR.
LINC00460 modulates KDM2A to promote cell proliferation and migration by targeting miR-342-3p in gastric cancer Fang Wang,1 Shaobo Liang,2 Xiaowei Liu,1 Lei Han,1 Junye Wang,1 Qin Du1 1Department of Oncology, Affiliated Hospital of Jining Medical College, Jining 272029, China; 2Department of Gastroenterology, Central Hospital of Shanxian County, Heze, 274399, China Background: Increasing.
The MTT assay is a colorimetric assay for assessing cell metabolic activity. NAD(P)H-dependent cellular oxidoreductase enzymes may, under defined conditions, reflect the number of viable cells present. These enzymes are capable of reducing the tetrazolium dye MTT 3-(4,5-di methyl thiazol-2-yl)-2,5-diphenyltetrazolium bromide to its insoluble formazan, which has a purple color.
Hep G2 cell line human has been used to study the cytotoxicity of carbon monoxide-releasing molecules and organic solvents on HepG2 cells and to evaluate the antiproliferative activity of aqueous extracellular polysaccharides (AEPS) for HepG2 using the MTT colorimetric assay. Study of hepatocyte function and specific protein expression.